Vanderbilt University Medical Center Research Assistant II in Nashville, Tennessee
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Located in Nashville, Tennessee, and operating at a global crossroads of teaching, discovery and patient care, VUMC is a community of individuals who come to work each day with the simple aim of changing the world. It is a place where your expertise will be valued, your knowledge expanded and your abilities challenged. It is a place where your diversity — of culture, thinking, learning and leading — is sought and celebrated. It is a place where employees know they are part of something that is bigger than themselves, take exceptional pride in their work and never settle for what was good enough yesterday. Vanderbilt’s mission is to advance health and wellness through preeminent programs in patient care, education, and research.
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The RA II will conduct experiments and assays, maintain the laboratory and equipment and maintain inventories. A primary duty will be maintenance of the laboratory mouse colony, including breeding, weaning and genotyping, to support research the research of a vibrant lab with multiple research projects. The RA II will operate and maintain essential equipment, such as cell culture and plate readers as well as keep essential buffers and stock solutions in supply.
Conduct standard ELISA, coagulation, and fluorescent assays with oversight on both animal and human tissues
Proficient pipetting skills
Proficient at basic molecular biology assays including western blots, qPCR, standard PCR, and Bradford assays.
Proficient at basic cell culture techniques utilizing immortalized cell lines.
Proficient as basic scientific calculation for dilutions
Ordering of lab supplies
Washing and drying of lab glassware and plasticware
Work independently and troubleshoot assay problems with the help of a senior lab member
Able to organize, analyze, and present data to other members of the lab.
Proficient at presenting data in both a written and oral format to lab members
Proficient at analyzing primary literature to assist in assay design
Work with senior lab members to design assays with appropriate positive and negative controls
Maintain lab notes in an organized manner
Obtain, organize, and analyze human samples from various proved IRB studies
Organization and maintain of mouse colony
Organization of breeders, monitoring for litters, and updating litters in online catalog (mLims)
Weening of mice (ear tagging and cataloging in mLims)
Genotyping of mice and updating information in mLims
Standard mouse treatments (Paralube, antibotics, etc) as needed
Checking Mouse diets weekly
Assist lab members in administration of analgesic to mice following surgical procedures
Assist lab members in radiographic analysis of animals following injury and organize data.
Assist lab members in tissue collection following animal sacrifice
Assist lab members in locating previous animal samples for analysis
Washing and drying of lab glassware and plasticware
Autoclaving supplies as needed for surgical procedures
Organization of lab supplies and stocking of shelves in animal surgery bench
Keeping refrigerators, freezers, and mouse sample log up to date.
Preference but not necessary:
Experience with primary cellular culture techniques
Experience with flow cytometry
Excellent attention to detail
Work as a member of a team
Excellent organizational skills
Ability to prioritize tasks and optimize daily schedule with minimal oversight
VUMC Recent Accomplishments
Because we are committed to providing the best in patient care, education and research, we are proud of our recent accomplishments:
US News & World Report: #1 Hospital in Tennessee, #1 Health Care Provider in Nashville, #1 Audiology ( Bill Wilkerson Center at http://www.vanderbilthealth.com/billwilkerson/ ), 12 adult and 10 pediatric clinical specialties ranked among the nation's best, #15 Education and Training
Truven Health Analytics: among the top 50 cardiovascular hospitals in the U.S.
Becker's Hospital Review: one of the "100 Greatest Hospitals in America"
The Leapfrog Group: grade "A" in Hospital Safety Score
National Institutes of Health: among the top 10 grant awardees for medical research in the US
Magnet Recognition Program: Vanderbilt nurses are the only group honored in Tennessee
Nashville Business Journal: Middle Tennessee's healthiest employer
American Hospital Association: among the 100 "Most Wired" medical systems in the US
Bachelor's Degree (or equivalent experience) and 2 years experience
Physical Requirements/Strengths needed & Physical Demands:
Occasional: Sitting: Remaining in seated position
Occasional: Standing: Remaining on one's feet without moving.
Occasional: Walking: Moving about on foot.
Occasional: Lifting under 35 lbs: Raising and lowering objects under 35 lbs from one level to another
Occasional: Push/Pull: Exerting force to move objects away from or toward.
Occasional: Bending/Stooping: Trunk bending downward and forward by bending spine at waist requiring full use of lower extremities and back muscles
Occasional: Reaching below shoulders: Extending arms in any direction below shoulders.
Occasional: Handling: Seizing, holding, grasping, turning or otherwise working with hand or hands.
Occasional: Vision: Clarity of near vision at 20 inches or less and far vision at 20 feet or more with depth perception, peripheral vision, color vision.
Occasional: Noise: May include exposure to occupational noise levels which equal or exceed an 8-hr time-weighted average of 85 decibels, requiring enrollment in VUMC's Hearing Conservation Program which includes training, use of hearing protection, and periodic audiometry.
Continuous: Communication: Expressing or exchanging written/verbal/electronic information.
Occasional: Chemicals and Gasses: Medications, cleaning chemicals, oxygen, other medical gases used in work area.
Occasional: Pathogens: Risk of exposure to bloodborne pathogens and other contagious illnesses.
Organization: Orthopaedic Surgery & Rehab 104475
Title: Research Assistant II
Location: TN-Nashville-Medical Center East (MCE) - South Tower
Requisition ID: 1805448