Vanderbilt University Medical Center Research Assistant II in Nashville, Tennessee

Discover Vanderbilt University Medical Center:

Located in Nashville, Tennessee, and operating at a global crossroads of teaching, discovery and patient care, VUMC is a community of individuals who come to work each day with the simple aim of changing the world. It is a place where your expertise will be valued, your knowledge expanded and your abilities challenged. It is a place where your diversity — of culture, thinking, learning and leading — is sought and celebrated. It is a place where employees know they are part of something that is bigger than themselves, take exceptional pride in their work and never settle for what was good enough yesterday. Vanderbilt’s mission is to advance health and wellness through preeminent programs in patient care, education, and research.

Click Here To View The VUMC Promise of Discovery


The RA II will conduct experiments and assays, maintain the laboratory and equipment and maintain inventories. A primary duty will be maintenance of the laboratory mouse colony, including breeding, weaning and genotyping, to support research the research of a vibrant lab with multiple research projects. The RA II will operate and maintain essential equipment, such as cell culture and plate readers as well as keep essential buffers and stock solutions in supply.

  • Conduct standard ELISA, coagulation, and fluorescent assays with oversight on both animal and human tissues

  • Proficient pipetting skills

  • Proficient at basic molecular biology assays including western blots, qPCR, standard PCR, and Bradford assays.

  • Proficient at basic cell culture techniques utilizing immortalized cell lines.

  • Proficient as basic scientific calculation for dilutions

  • Ordering of lab supplies

  • Washing and drying of lab glassware and plasticware

  • Work independently and troubleshoot assay problems with the help of a senior lab member

  • Able to organize, analyze, and present data to other members of the lab.

  • Proficient at presenting data in both a written and oral format to lab members

  • Proficient at analyzing primary literature to assist in assay design

  • Work with senior lab members to design assays with appropriate positive and negative controls

  • Maintain lab notes in an organized manner

  • Obtain, organize, and analyze human samples from various proved IRB studies

Mouse colony:

  • Organization and maintain of mouse colony

  • Organization of breeders, monitoring for litters, and updating litters in online catalog (mLims)

  • Weening of mice (ear tagging and cataloging in mLims)

  • Genotyping of mice and updating information in mLims

  • Standard mouse treatments (Paralube, antibotics, etc) as needed

  • Checking Mouse diets weekly

  • Assist lab members in administration of analgesic to mice following surgical procedures

  • Assist lab members in radiographic analysis of animals following injury and organize data.

  • Assist lab members in tissue collection following animal sacrifice

  • Assist lab members in locating previous animal samples for analysis

  • Washing and drying of lab glassware and plasticware

  • Autoclaving supplies as needed for surgical procedures

  • Organization of lab supplies and stocking of shelves in animal surgery bench

  • Keeping refrigerators, freezers, and mouse sample log up to date.

  • Preference but not necessary:

  • Experience with primary cellular culture techniques

  • Experience with flow cytometry

  • Excellent attention to detail

  • Work as a member of a team

  • Excellent organizational skills

  • Self-driven

  • Ability to prioritize tasks and optimize daily schedule with minimal oversight

VUMC Recent Accomplishments

Because we are committed to providing the best in patient care, education and research, we are proud of our recent accomplishments:

  • US News & World Report: #1 Hospital in Tennessee, #1 Health Care Provider in Nashville, #1 Audiology ( Bill Wilkerson Center at ), 12 adult and 10 pediatric clinical specialties ranked among the nation's best, #15 Education and Training

  • Truven Health Analytics: among the top 50 cardiovascular hospitals in the U.S.

  • Becker's Hospital Review: one of the "100 Greatest Hospitals in America"

  • The Leapfrog Group: grade "A" in Hospital Safety Score

  • National Institutes of Health: among the top 10 grant awardees for medical research in the US

  • Magnet Recognition Program: Vanderbilt nurses are the only group honored in Tennessee

  • Nashville Business Journal: Middle Tennessee's healthiest employer

  • American Hospital Association: among the 100 "Most Wired" medical systems in the US

Bachelor's Degree (or equivalent experience) and 2 years experience

Physical Requirements/Strengths needed & Physical Demands:


  • Occasional: Sitting: Remaining in seated position

  • Occasional: Standing: Remaining on one's feet without moving.

  • Occasional: Walking: Moving about on foot.

  • Occasional: Lifting under 35 lbs: Raising and lowering objects under 35 lbs from one level to another

  • Occasional: Push/Pull: Exerting force to move objects away from or toward.

  • Occasional: Bending/Stooping: Trunk bending downward and forward by bending spine at waist requiring full use of lower extremities and back muscles

  • Occasional: Reaching below shoulders: Extending arms in any direction below shoulders.

  • Occasional: Handling: Seizing, holding, grasping, turning or otherwise working with hand or hands.


  • Occasional: Vision: Clarity of near vision at 20 inches or less and far vision at 20 feet or more with depth perception, peripheral vision, color vision.

  • Occasional: Noise: May include exposure to occupational noise levels which equal or exceed an 8-hr time-weighted average of 85 decibels, requiring enrollment in VUMC's Hearing Conservation Program which includes training, use of hearing protection, and periodic audiometry.

  • Continuous: Communication: Expressing or exchanging written/verbal/electronic information.

Environmental Conditions

  • Occasional: Chemicals and Gasses: Medications, cleaning chemicals, oxygen, other medical gases used in work area.

  • Occasional: Pathogens: Risk of exposure to bloodborne pathogens and other contagious illnesses.

Job Research

Organization: Orthopaedic Surgery & Rehab 104475

Title: Research Assistant II

Location: TN-Nashville-Medical Center East (MCE) - South Tower

Requisition ID: 1805448