Vanderbilt University Medical Center Research Asst II-Pediatric Pulmonary-FT Days in Nashville, Tennessee
The Division of Pulmonary Medicine in the Department of Pediatrics is seeking a full-time Research Assistant to join a productive laboratory to assist with research on the cell biology of pulmonary fibrosis and genetic lung diseases in adults and children.
The position will require performance of a combination of molecular and cell biology as well as designated work in maintaining and performing experiments using transgenic mouse colonies.
Under moderate supervision the Research Assistant II will:
Understand and perform routine procedures including mouse colony management and standard PCR.
Perform real time PCR, western blotting, ELISA, immunostaining, and cell culture.
Become proficient with tissue dissection/animal injections.
Become proficient in proper processing and catologing of human samples for genetic, genomic, and proteomic research
Assist in other laboratory and research functions.
Key Functions and Expected Performances:
1. Under moderate supervision:
Understand and follow experimental protocols, including animal handling, cell isolation and tissue harvest, cell culture, and molecular assays
Set up, conduct experiments, and continue research projects following protocols
Prepare and operate equipment in experimental procedures and maintain laboratory inventory and equipment
Follow University safety protocols, and laboratory protocols including maintaining sterile conditions as required
Maintain inventories and process and catalog specimens
Perform accurate calculations in the preparation of reagents and solutions
Keep detailed documentation of all procedures and protocols in a lab notebook and electronically as appropriate
Compile and communicate results to PI and lab manager, including the appropriate recording of data and reporting of standard or variant results
Recognize and communicate potential problems and suggest solutions
Bachelor’s degree plus minimum 24 months experience; Masters Degree preferred
Basic laboratory skills and prior molecular biology laboratory experience is required.
A degree in Biochemistry, Cell Biology or related science field is preferred.
Previous research experience working with mice is preferred.
Required skills: strong analytical and communication skills; the ability to work independently; computer skills including word processing, data entry, statistical analysis, and graphic presentation.
Bachelor's Degree (or equivalent experience) and 2 years experience
Licensure, Certification, and/or Registration (LCR):
Physical Requirements/Strengths needed & Physical Demands:
Occasional: Sitting: Remaining in seated position
Occasional: Standing: Remaining on one's feet without moving.
Occasional: Walking: Moving about on foot.
Occasional: Lifting under 35 lbs: Raising and lowering objects under 35 lbs from one level to another
Occasional: Push/Pull: Exerting force to move objects away from or toward.
Occasional: Bending/Stooping: Trunk bending downward and forward by bending spine at waist requiring full use of lower extremities and back muscles
Occasional: Reaching below shoulders: Extending arms in any direction below shoulders.
Occasional: Handling: Seizing, holding, grasping, turning or otherwise working with hand or hands.
Occasional: Vision: Clarity of near vision at 20 inches or less and far vision at 20 feet or more with depth perception, peripheral vision, color vision.
Occasional: Noise: May include exposure to occupational noise levels which equal or exceed an 8-hr time-weighted average of 85 decibels, requiring enrollment in VUMC's Hearing Conservation Program which includes training, use of hearing protection, and periodic audiometry.
Continuous: Communication: Expressing or exchanging written/verbal/electronic information.
Occasional: Chemicals and Gasses: Medications, cleaning chemicals, oxygen, other medical gases used in work area.
Occasional: Pathogens: Risk of exposure to bloodborne pathogens and other contagious illnesses.
Organization: Pediatric Pulmonary 104621
Title: Research Asst II-Pediatric Pulmonary-FT Days
Location: TN-Nashville-DOT - VCH Doctor's Office Tower
Requisition ID: 1807311